A UV-Vis spectrophotometer is an instrument used to measure the absorption of ultraviolet (UV) and visible light by a sample. It is a valuable tool in various scientific fields, including chemistry, biochemistry, and materials science.
Description
How it Works
Light Source: The spectrophotometer generates a beam of light that spans the UV and visible wavelengths.
Sample Cell: The sample is placed in a transparent cell that allows the light to pass through.
Monochromator: The light is dispersed into its individual wavelengths using a monochromator.
Detector: The detector measures the intensity of the light that passes through the sample.
Absorption Calculation: The spectrophotometer calculates the absorbance of the sample based on the difference between the intensity of the incident light and the transmitted light.
Data Analysis: The absorbance data is analyzed to determine the concentration of the analyte in the sample, using Beer-Lambert Law.
Types of UV-Vis Spectrophotometers
Single-Beam Spectrophotometer: Uses a single light source and detector.
Double-Beam Spectrophotometer: Uses two light sources and detectors, allowing for simultaneous measurement of the sample and a reference.
